DUOX1 Antibody (OAEB00847)

Catalog No: OAEB00847 (Formerly GWB-621F66)

Size:100UG

OAEB00847 (4ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Additional Information: Epitope Info: internal region
  Immunogen: Peptide with sequence EKFQRSCLHQTVQ, from the internal region of the protein sequence according to NP_059130.2 ; NP_787954.1.
  Note: This antibody is expected to recognise both reported isoforms (NP_059130.2 ; NP_787954.1) which are identical;
  Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: DUOX1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Peptide Sequence: EKFQRSCLHQTVQ
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:32000.
  Immunohistochemistry: In paraffin embedded Human Thyroid shows staining of cytoplasm in epithelial cells of the gland. Recommended concentration, 4-6ug/ml.
  Western Blot: Preliminary experiments in Human Placenta and Thyroid Gland lysates gave no specific signal but low background (at antibody concentration up to 0.5ug/ml).
• Reference: Harper RW, Xu C, Eiserich JP, Chen Y, Kao CY, Thai P, Setiadi H, Wu R.
  Differential regulation of dual NADPH oxidases/peroxidases, Duox1 and Duox2, by Th1 and Th2 cytokines in respiratory tract epithelium.
  FEBS Lett. 2005 Aug 29;579(21):4911-7. PMID: 16111680

Target Info

• Gene Symbol: DUOX1
• Gene Full Name: dual oxidase 1
• Alias Symbols: dual oxidase 1;flavoprotein NADPH oxidase;large NOX 1;LNOX1;long NOX 1;NADPH thyroid oxidase 1;nicotinamide adenine dinucleotide phosphate oxidase;NOXEF1;THOX1;thyroid oxidase 1.
• NCBI Gene Id: 53905
• Protein Name: Dual oxidase 1 EMBL AAI14939.1
• Uniprot ID: Q14C85
• Protein Accession #: NP_059130.2
• Molecular Weight: 177