DSTN ELISA Kit (Human) (OKCD00558)

Catalog No: OKCD00558 (Formerly GWB-KBBNF7)

Size:96WELLS

Human DSTN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	96-104%	84-105%	92-105%	80-94%
  EDTA Plasma (n=5)	80-101%	97-105%	78-94%	87-104%
  Heparin Plasma (n=5)	99-105%	93-101%	80-89%	94-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Destrin (DSTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Destrin (DSTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Destrin (DSTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Destrin (DSTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	102
  EDTA plasma(n=5)	80-93	88
  heparin plasma(n=5)	78-96	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Destrin (DSTN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Destrin (DSTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DSTN Microplate: 96 Wells (12 x 8 Well Strips)
  • DSTN Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated DSTN Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Destrin (DSTN). No significant cross-reactivity or interference between Destrin (DSTN) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DSTN
• Gene Full Name: destrin, actin depolymerizing factor
• Alias Symbols: ACTDP;actin-depolymerizing factor;ADF;bA462D18.2;bA462D18.2 (destrin (actin depolymerizing factor ADF) (ACTDP));destrin;epididymis luminal protein 32;epididymis secretory sperm binding protein;HEL32.
• NCBI Gene Id: 11034
• Protein Name: Destrin
• Description of Target: Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.
• Uniprot ID: P60981
• Protein Accession #: NP_001011546
• Nucleotide Accession #: NM_001011546.1
• Protein Size (# AA): 165