Catalog No: OKCD08131
Size:96WELLS
Price: $800.00
SKU
OKCD08131
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for DSPP ELISA Kit (Human) (OKCD08131) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dentin Sialophosphoprotein (DSPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dentin Sialophosphoprotein (DSPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dentin Sialophosphoprotein (DSPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dentin Sialophosphoprotein (DSPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dentin Sialophosphoprotein (DSPP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dentin Sialophosphoprotein (DSPP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.58ng/mL |
Gene Symbol | DSPP |
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Gene Full Name | dentin sialophosphoprotein |
Alias Symbols | deafness, autosomal dominant 39;dentin phosphophoryn;dentin phosphoprotein;dentin phosphoryn;dentin sialophosphoprotein;dentin sialoprotein;DFNA39;DGI1;DMP3;DPP;DSP. |
NCBI Gene Id | 1834 |
Protein Name | Dentin sialophosphoprotein |
Description of Target | DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals. |
Uniprot ID | Q9NZW4 |
Protein Accession # | NP_055023.2 |
Nucleotide Accession # | NM_014208.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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