Catalog No: OKEH05480
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Dsg2 ELISA Kit (Mouse) (OKEH05480) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dsg2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dsg2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dsg2 captured in the well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 103%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <6.9%, n = 20
Mean Inter-Assay Precision - <8.8%, n = 20
ELISA Kit Component
DSG2 Microplate96 Wells (12 x 8 Well strips)
DSG2 Lyophilized Standard2
100X Biotinylated DSG2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.76 pg/mL
SpecificityNatural and recombinant Mouse Desmoglein-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDsg2
Gene Full Namedesmoglein 2
Alias SymbolsAA408168;D18Ertd293;D18Ertd293e;desmoglein-2.
NCBI Gene Id13511
Protein NameO55111
Description of TargetDesmoglein-2
Uniprot IDhttps://www.uniprot.org/uniprot/O55111
Protein Accession #NP_031909.2
Nucleotide Accession #NM_007883.3
Protein Size (# AA)1122
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