Catalog No: OKCD00505 (Formerly GWB-KBBEK3)
Size:96WELLS
Price: $675.00
SKU
OKCD00505
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Datasheets/ManualsPrintable datasheet for DSC1 ELISA Kit (Human) (OKCD00505)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmocollin 1 (DSC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Desmocollin 1 (DSC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Desmocollin 1 (DSC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Desmocollin 1 (DSC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Desmocollin 1 (DSC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Desmocollin 1 (DSC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DSC1 Microplate96 Wells (12 x 8 Well Strips)
DSC1 Lyophilized Standard2 x 10 ng
100X Biotinylated DSC1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Desmocollin 1 (DSC1). No significant cross-reactivity or interference between Desmocollin 1 (DSC1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDSC1
Gene Full Namedesmocollin 1
Alias Symbolscadherin family member 1;CDHF1;desmocollin-1;desmosomal glycoprotein 2/3;DG2/DG3.
NCBI Gene Id1823
Protein NameDesmocollin-1
Description of TargetComponent of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Linked to the keratinization of epithelial tissues.
Uniprot IDQ08554
Protein Accession #NP_004939
Nucleotide Accession #NM_004948.3
Protein Size (# AA)894
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