DROSHA ELISA Kit (Human) (OKCD00897)

Catalog No: OKCD00897 (Formerly GWB-KBBJI8)

Size:96WELLS

Human DROSHA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease III, Nuclear (RNASEN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease III, Nuclear (RNASEN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease III, Nuclear (RNASEN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ribonuclease III, Nuclear (RNASEN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonuclease III, Nuclear (RNASEN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonuclease III, Nuclear (RNASEN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DROSHA Microplate: 96 Wells (12 x 8 Well Strips)
  • DROSHA Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated DROSHA Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.126 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ribonuclease III, Nuclear (RNASEN). No significant cross-reactivity or interference between Ribonuclease III, Nuclear (RNASEN) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DROSHA
• Gene Full Name: drosha ribonuclease III
• Alias Symbols: drosha, double-stranded RNA-specific endoribonuclease;ETOHI2;HSA242976;nuclear RNase III Drosha;p241;Protein Drosha;putative protein p241 which interacts with transcription factor Sp1;putative ribonuclease III;RANSE3L;ribonuclease 3;Ribonuclease III;ribonuclease type III, nuclear;RN3;RNase III;RNASE3L;RNASEN.
• NCBI Gene Id: 29102
• Protein Name: Ribonuclease 3
• Description of Target: Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
• Uniprot ID: Q9NRR4
• Protein Accession #: NP_001093882
• Nucleotide Accession #: NM_001100412.1
• Protein Size (# AA): 1374