Size:96 Wells
$550.00
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SKU
OKEI00758
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DRD3 ELISA Kit (Rat) (OKEI00758)

Catalog#: OKEI00758
Domestic: within 1-2 weeks delivery International: 1-2 weeks
More Information
Predicted Species Reactivity Rat
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology Drd3 ELISA Kit (Rat) (OKEI00758) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Drd3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Drd3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Drd3
Kit Range 0.313 - 20 ng/mL
Kit Reproducibility Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Drd3. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Drd3 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Component
ComponentAmount
Drd3 Microplate96 Wells (12 x 8 Well strips)
Drd3 Lyophilized Standard2
100X Drd3 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
30X Wash Buffer1 x 30 mL
100X Luminol Substrate1 x 10 mL
Substrate Diluent1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Biological Fluids
Sensitivity 0.188 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol DRD3
Alias Symbols D(3) dopamine receptor, Dopamine D3 receptor
NCBI Gene Id 29238
Protein Name D(3) dopamine receptor
Description of Target Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.
Swissprot Id P19020
Protein Accession # NP_058836
Nucleotide Accession # NM_017140
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express DRD3.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express DRD3.
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