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DRD3 ELISA Kit (Rat) (OKEI00758)

96 Wells
$550.00
In Stock
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Predicted Species Reactivity:
Rat
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology Drd3 ELISA Kit (Rat) (OKEI00758) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Drd3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Drd3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Drd3
Kit Range:
0.313 - 20 ng/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Drd3. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Drd3 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Component:
ComponentAmount
Drd3 Microplate96 Wells (12 x 8 Well strips)
Drd3 Lyophilized Standard2
100X Drd3 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
30X Wash Buffer1 x 30 mL
100X Luminol Substrate1 x 10 mL
Substrate Diluent1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
DRD3
Alias Symbols:
D(3) dopamine receptor, Dopamine D3 receptor
NCBI Gene Id:
29238
Protein Name:
D(3) dopamine receptor
Description of Target:
Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.
Swissprot Id:
P19020
Protein Accession #:
NP_058836
Nucleotide Accession #:
NM_017140
Sample Type:
Serum, Plasma, Biological Fluids
Sensitivity:
0.188 ng/mL
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express DRD3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express DRD3.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA

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