DR1 Antibody - N-terminal region (ARP38085_T100)

Catalog#: ARP38085_T100

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Replacement Item: This antibody may replace item sc-133522 from Santa Cruz Biotechnology.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DR1
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: MASSSGNDDDLTIPRAAINKMIKETLPNVRVANDARELVVNCCTEFIHLI
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Blocking Peptide: For anti-DR1 (ARP38085_T100) antibody is Catalog # AAP38085 (Previous Catalog # AAPP20259)
• Target Reference: Gilfillan,S., et al., (2005) J. Biol. Chem. 280 (7), 6222-6230
• Publications:

  Deng, W., Malecová, B., Oelgeschläger, T. & Roberts, S. G. E. TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation. Mol. Cell. Biol. 29, 1389-400 (2009). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 19114554

• Gene Symbol: DR1
• Official Gene Full Name: Down-regulator of transcription 1, TBP-binding (negative cofactor 2)
• Alias Symbols: NC2, NC2-BETA
• NCBI Gene Id: 1810
• Protein Name: Protein Dr1
• Description of Target: DR1 is a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. DR1 is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. DR1 contains a histone fold motif
• Swissprot Id: Q01658
• Protein Accession #: NP_001929
• Nucleotide Accession #: NM_001938
• Protein Size (# AA): 176
• Molecular Weight: 19kDa
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express DR1.
• Protein Interactions: POLE3; TBP; UBC; BTF3L4; LRSAM1; GOPC; NPLOC4; IPO11; DBNL; ARIH1; TTLL12; ARPC1B; UBE3A; METTL1; HSF1; XPO1; YEATS2; DRAP1; CREB1; CCDC101; TADA2B; SUPT20H; MBIP; WDR5; TADA3; KAT2B; MAP3K7; TADA2A; KAT2A; DR1; ELAVL1; NFYC; NFIL3; TTF2; IRF1;

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: DR1 antibody tested with Human Transfected 293T Cells (ARP38085_T100)