Catalog No: OKCA02371
Size:96 Wells
Price: $754.00
SKU
OKCA02371
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat10088-104
1:29790-101
1:49486-98
1:89993-103
ELISA Kit PrincipleAviva Systems Biology Dpysl2 ELISA Kit (Mouse) (OKCA02371) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dpysl2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dpysl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Dpysl2 captured in the well.
ELISA Kit Range31.25 - 2,000 pg/mL
ELISA Kit Recovery
Sample TypeMean Recovery (%)Range (%)
Serum (n=5)9184-95
EDTA Plasma (n=4)10293-106
ELISA Kit ReproducibilityMean Intra-Assay: CV <12% (n=20)
Mean Inter-Assay: CV <15% (n=24)
ELISA Kit Component
ComponentAmount
Dpysl2 Microplate96 Wells (12 x 8 Well strips)
Dpysl2 Lyophilized Standard2
100X Biotinylated Dpysl2 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity7.81 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDPYSL2
Alias SymbolsDR, Ul, Crm, DRP2, Crmp2, TOAD-, Ulip2, TOAD-64, AI851130, Musunc33
NCBI Gene Id12934
Protein NameDihydropyrimidinase-related protein 2
Description of TargetPlays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.
Uniprot IDO08553
Protein Accession #NP_034085
Nucleotide Accession #NM_009955
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