DPYD ELISA Kit (Mouse) (OKCD02507)

Catalog No: OKCD02507

Size:96WELLS

Mouse DPYD ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidine Dehydrogenase (DPYD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydropyrimidine Dehydrogenase (DPYD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidine Dehydrogenase (DPYD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dihydropyrimidine Dehydrogenase (DPYD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dihydropyrimidine Dehydrogenase (DPYD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dihydropyrimidine Dehydrogenase (DPYD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DPYD Microplate: 96 Wells (12 x 8 Well strips)
  • DPYD Lyophilized Standard: 2
  • 100X Biotinylated DPYD Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.054 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dihydropyrimidine Dehydrogenase (DPYD). No significant cross-reactivity or interference between Dihydropyrimidine Dehydrogenase (DPYD) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Dpyd
• Gene Full Name: dihydropyrimidine dehydrogenase
• Alias Symbols: AI315208;DHPDHase;dihydropyrimidine dehydrogenase [NADP(+)];dihydrothymine dehydrogenase;dihydrouracil dehydrogenase;DP;DPD;E330028L06Rik.
• NCBI Gene Id: 99586
• Protein Name: Dihydropyrimidine dehydrogenase [NADP(+)]
• Description of Target: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine (By similarity).
• Uniprot ID: Q8CHR6
• Protein Accession #: NP_740748.1
• Nucleotide Accession #: NM_170778.2
• Protein Size (# AA): 1025