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Catalog No: OKCD02503
Size:96WELLS
Price: $700.00
SKU
OKCD02503
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Datasheets/ManualsPrintable datasheet for DPP9 ELISA Kit (Mouse) (OKCD02503)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase 9 (DPP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidyl Peptidase 9 (DPP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase 9 (DPP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dipeptidyl Peptidase 9 (DPP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78.12-5000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dipeptidyl Peptidase 9 (DPP9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dipeptidyl Peptidase 9 (DPP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DPP9 Microplate96 Wells (12 x 8 Well strips)
DPP9 Lyophilized Standard2
100X Biotinylated DPP9 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity25 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dipeptidyl Peptidase 9 (DPP9). No significant cross-reactivity or interference between Dipeptidyl Peptidase 9 (DPP9) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolDpp9
Gene Full Namedipeptidylpeptidase 9
Alias Symbols6430584G11Rik;A330078I11;dipeptidyl peptidase 9;dipeptidyl peptidase IX;dipeptidyl peptidase-like protein 9;DP;DP9;DPLP9;DPP IX;DPRP2.
NCBI Gene Id224897
Protein NameDipeptidyl peptidase 9
Description of TargetDipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2.
Uniprot IDQ8BVG4
Protein Accession #NP_766212.2
Nucleotide Accession #NM_172624.3
Protein Size (# AA)862
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