DPP6 ELISA Kit (Human) (OKCD01789)

Catalog No: OKCD01789 (Formerly GWB-KBBRU7)

Size:96WELLS

Human DPP6 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	83-92%	98-105%	88-102%	82-98%
  EDTA Plasma (n=5)	79-105%	89-96%	93-101%	84-98%
  Heparin Plasma (n=5)	98-105%	78-101%	86-104%	97-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase 6 (DPP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidyl Peptidase 6 (DPP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase 6 (DPP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dipeptidyl Peptidase 6 (DPP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	83-99	88
  EDTA Plasma (n=5)	83-102	87
  Heparin Plasma (n=5)	83-99	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dipeptidyl Peptidase 6 (DPP6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dipeptidyl Peptidase 6 (DPP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DPP6 Microplate: 96 Wells (12 x 8 Well Strips)
  • DPP6 Lyophilized Standard: 2 x 200 ng
  • 100X Biotinylated DPP6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 1.08 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dipeptidyl Peptidase 6 (DPP6). No significant cross-reactivity or interference between Dipeptidyl Peptidase 6 (DPP6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DPP6
• Gene Full Name: dipeptidyl peptidase like 6
• Alias Symbols: dipeptidyl aminopeptidase IV-related protein;dipeptidyl aminopeptidase-like protein 6;Dipeptidyl aminopeptidase-related protein;Dipeptidyl peptidase 6;Dipeptidyl peptidase IV-like protein;dipeptidyl peptidase IV-related protein;dipeptidyl peptidase VI;dipeptidyl-peptidase 6;DPL1;DPP VI;DPPX;MRD33;VF2.
• NCBI Gene Id: 1804
• Protein Name: Dipeptidyl aminopeptidase-like protein 6
• Description of Target: Promotes cell surface expression of the potassium channel KCND2 (PubMed:15454437, PubMed:19441798). Modulates the activity and gating characteristics of the potassium channel KCND2 (PubMed:18364354). Has no dipeptidyl aminopeptidase activity (PubMed:8103397, PubMed:15476821).
• Uniprot ID: P42658
• Protein Accession #: NP_570629.2
• Nucleotide Accession #: NM_130797.3
• Protein Size (# AA): 865