DPP10 ELISA Kit (Human) (OKEH04330)

Catalog No: OKEH04330

Size:96T

Human DPP10 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology DPP10 ELISA Kit (Human) (OKEH04330) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DPP10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DPP10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DPP10 captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.2% (n = 20)
  Mean Inter-assay CV%: <= 9.8% (n = 20)
• ELISA Kit Component:
  • DPP10 Microplate: 96 Wells (12 x 8 Well Strips)
  • DPP10 Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • DPP10 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.15 ng/mL
• Specificity: Natural and recombinant Human Inactive dipeptidyl peptidase 10
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DPP10
• Gene Full Name: dipeptidyl peptidase like 10
• Alias Symbols: dipeptidyl peptidase 10;dipeptidyl peptidase IV-related protein 3;dipeptidyl peptidase X;dipeptidyl peptidase-like protein 2;dipeptidyl-peptidase 10 (inactive);dipeptidyl-peptidase 10 (non-functional);DPL2;DPP X;DPPY;DPRP3;DPRP-3;inactive dipeptidyl peptidase 10.
• NCBI Gene Id: 57628
• Protein Name: Q8N608
• Description of Target: Inactive dipeptidyl peptidase 10
• Uniprot ID: https://www.uniprot.org/uniprot/Q8N608
• Protein Accession #: NP_001004360.2
• Nucleotide Accession #: NM_001004360.3
• Protein Size (# AA): 796
• Molecular Weight: 91 kDa