Catalog No: OKEH06578
Size:96 Wells
Price: $675.00
SKU
OKEH06578
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityDog
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Cdh1 ELISA Kit (Dog) (OKEH06578) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdh1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cdh1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdh1 captured in the well.
ELISA Kit Range0.156 - 10ng/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Cdh1 Microplate96 Wells (12 x 8 Well strips)
Cdh1 Lyophilized Standard2 x 10 ng
100X Cdh1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.052 ng/mL
SpecificityNatural and recombinant Dog Cadherin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCDH1
Gene Full Namedipeptidase 1 (renal)
Alias SymbolsCadherin-1, Uvomorulin
NCBI Gene Id442858
Protein NameCadherin-1
Description of TargetCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Swissprot IdF1PAA9
Protein Accession #NP_001274054
Nucleotide Accession #NM_001287125
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CDH1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CDH1.
Write Your Own Review
You're reviewing:CDH1 ELISA Kit (Dog) (OKEH06578)
Your Rating
We found other products you might like!