DPAGT1 ELISA Kit (Human) (OKCD09162)

Catalog No: OKCD09162

Size:96WELLS

DPAGT1 ELISA Kit (Human) (OKCD09162) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dolichyl Phosphate-N-Acetylglucosaminephosphotransferase 1 (DPAGT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • DPAGT1 Microplate: 96 Wells (12 x 8 Well strips)
  • DPAGT1 Lyophilized Standard: 2
  • 100X DPAGT1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.059ng/mL

Target Info

• Gene Symbol: DPAGT1
• Gene Full Name: dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
• Alias Symbols: GPT, ALG7, DGPT, G1PT, UAGT, UGAT, CDG1J, CMS13, DPAGT, CDG-Ij, CMSTA2, DPAGT2, D11S366
• NCBI Gene Id: 1798
• Protein Name: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
• Description of Target: The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme.
• Uniprot ID: Q9H3H5
• Protein Accession #: NP_001373.2
• Nucleotide Accession #: NM_001382.3