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Catalog No: OKCD04272
Size:96WELLS
Price: $750.00
SKU
OKCD04272
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Datasheets/ManualsPrintable datasheet for DOT1L ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DOT1 Like, Histone H3 Methyltransferase (DOT1L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DOT1 Like, Histone H3 Methyltransferase (DOT1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DOT1 Like, Histone H3 Methyltransferase (DOT1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DOT1 Like, Histone H3 Methyltransferase (DOT1L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)78-9990
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DOT1 Like, Histone H3 Methyltransferase (DOT1L) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DOT1 Like, Histone H3 Methyltransferase (DOT1L) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Dot1l Microplate96 Wells (12 x 8 Well strips)
Dot1l Lyophilized Standard2
100X Dot1l HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity0.063 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDOT1L
Alias SymbolsDot1, KMT4, mDot, mDot1, AW907654, A630076O07
NCBI Gene Id208266
Protein Namehistone-lysine N-methyltransferase, H3 lysine-79 specific
Uniprot IDQ6XZL8
Protein Accession #NP_955354.1
Nucleotide Accession #NM_199322.1
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