Dog IgG (H&L) Antibody - HRP Conjugated (OARA04896)

Catalog No: OARA04896

Size:2mg

Product Info

• Product Format: Lyophilized
• Isotype: IgG
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IHC
• Additional Information: Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Color: Substrate dependent
  Conjugation Reference: Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
  Label: Peroxidase (Horseradish)
  Preservative: 0.01% (w/v) Gentamicin Sulfate (Do NOT add Sodium Azide!)
  Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Reconstitution and Storage: Store vial at 4 C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Immunogen: Dog IgG whole molecule
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against antiPeroxidase, anti-Rabbit Serum, Dog IgG and Dog Serum
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Application Info: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  ELISA: 1:165,000
  WB: 1:3,000 - 1:5,000
  IHC: 1:1,000-1:5,000

Target Info

• Molecular Weight: 40000