Goat Anti-Dog IgG F(c) Polyclonal Antibody - HRP Conjugated (OARA04920)

Catalog No: OARA04920

Size:2MG

Product Info

• Predicted Species Reactivity: Dog
• Product Format: Lyophilized
• Clonality: Polyclonal
• Isotype: IgG
• Host: Goat
• Conjugation: HRP
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
  Immunogen Type: Native
• Reconstitution and Storage: 2°C to 8°C|-20°C or -80°C
• Immunogen: Anti-Dog IgG F(c) whole molecule was produced by repeated immunization with Dog IgG F(c) whole molecule in goat.
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Concentration: 2 mg/ml
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Dog IgG, Dog IgG F(c) and Dog Serum. No reaction was observed against Dog IgG F(ab).
• Application Info: ELISA: 1:10000~50000
  IHC: 1:500~2500
  WB: 1:1000~10000
• Application Data: This secondary antibody anti-Dog is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Target Info

• Alias Symbols: HRP
• Description of Target: Anti-Dog IgG F(c) whole molecule antibody generated in goat detects specifically Dog IgG F(c) whole molecule. It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of dog IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
• Molecular Weight: 40 kDa