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Catalog No: OASE00189 (Formerly GWB-74D810)
Size:100ug
Price: $429.00
SKU
OASE00189
Availability: Discontinued
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Datasheets/ManualsPrintable datasheet for OASE00189
Product Info
Predicted Species ReactivityHuman, Mouse
ClonalityMonoclonal
CloneS117-9
IsotypeIgG1
HostMouse
ConjugationUnconjugated
ApplicationWB, IHC, ICC, IF, IP, AM
Additional InformationBackground Info: Detects ~45kDa. No cross-reactivity against DNMT3a or DNMT3B.
::Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation
of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic
activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).
::Certificate of Analysis: 1ug/mL was sufficient for detection of DNMT3L in 10ug of COS cell transiently transfected with Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Reconstitution and StorageStore at -20C. Shipping with Blue Ice or 4C.
ImmunogenFusion protein amino acids 1-387 of human DNMT3L
PurificationProtein G Purified
Concentration1 mg/ml
SpecificityDetects ~45kDa. No cross-reactivity against DNMT3a or DNMT3B.
DilutionWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Storage BufferPBS pH7.4, 50% glycerol, 0.09% sodium azide
Reference1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983.
2. Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl.
Acids Res. 20: 2287-2291.
3. Xie S. et al. (1999) Gene 236: 87-95.
4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99:
247-257.
5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382.
6. Robertson K.D., et al. (2000) Nat Genet 25(3):338-342.
7. Kareta MS., et al. (2006) J Biol Chem. 281(36): 25893-
25902.
8. Gokul G., Ramakrishna G., and Khosla S. (2009)
Epigenetics. 4(5): 322-329.
DescriptionMethylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).
Gene SymbolDNMT3L
Gene Full NameDNA (cytosine-5-)-methyltransferase 3-like
Alias SymbolsCytosine 5 methyltransferase 3 like protein, DNA (cytosine 5 ) methyltransferase 3 like, DNA (cytosine-5)-methyltransferase 3-like, DNA cytosine 5 methyltransferase 3 like protein, DNA methyltransferase 3 like protein, DNM3L_HUMAN, Dnmt 3L, Dnmt3l, Human cytosine 5 methyltransferase 3 like protein, MGC1090
NCBI Gene Id29947
Uniprot IDQ9UJW3
Protein Accession #NP_037501.2
  1. What is the species homology for "Dnmt3L Antibody (OASE00189)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".

  2. How long will it take to receive "Dnmt3L Antibody (OASE00189)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Dnmt3L Antibody (OASE00189)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Dnmt3L Antibody (OASE00189)"?

    This target may also be called "Cytosine 5 methyltransferase 3 like protein, DNA (cytosine 5 ) methyltransferase 3 like, DNA (cytosine-5)-methyltransferase 3-like, DNA cytosine 5 methyltransferase 3 like protein, DNA methyltransferase 3 like protein, DNM3L_HUMAN, Dnmt 3L, Dnmt3l, Human cytosine 5 methyltransferase 3 like protein, MGC1090" in publications.

  5. What is the shipping cost for "Dnmt3L Antibody (OASE00189)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Dnmt3L Antibody (OASE00189)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Dnmt3L Antibody (OASE00189)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Dnmt3L Antibody (OASE00189)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "DNMT3L"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "DNMT3L"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "DNMT3L"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "DNMT3L"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "DNMT3L"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "DNMT3L"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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