DNMT3B Antibody - middle region (ARP49124_P050)

Catalog No: ARP49124_P050

WB Suggested Anti-DNMT3B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: OVCAR-3 cell lysateDNMT3B is supported by BioGPS gene expression data to be expressed in OVCAR3

Sample Type:
Lane 1: 20ug mouse mesenchymal stem cell lysate Primary Antibody Dilution:
1:2000 Secondary Antibody:
Anti-rabbit-HRP Secondary Antibody Dilution:
1:10,000 Color/Signal Descriptions:
DNMT3B Gene Name:
Anonymous Submitted by:

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DNMT3B
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: GTGRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAMKVGDKRDISRFLEC
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-DNMT3B (ARP49124_P050) antibody is Catalog # AAP49124 (Previous Catalog # AAPY02321)
• Sample Type Confirmation:

  DNMT3B is supported by BioGPS gene expression data to be expressed in OVCAR3

• Reference: Boland,M.J. (2008) J. Mol. Biol. 379 (3), 492-504

Target Info

• Gene Symbol: DNMT3B
• Gene Full Name: DNA (cytosine-5-)-methyltransferase 3 beta
• Alias Symbols: ICF, ICF1, M.HsaIIIB
• NCBI Gene Id: 1789
• Protein Name: DNA (cytosine-5)-methyltransferase 3B
• Description of Target: DNMT3B is required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. DNMT3B may preferentially methylate nucleosomal DNA within the nucleosome core region. DNMT3B may function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. DNMT3B seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, DNMT3B is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
• Uniprot ID: Q9UBC3-3
• Protein Accession #: NP_787045
• Nucleotide Accession #: NM_175849
• Protein Size (# AA): 770
• Molecular Weight: 86kDa
• Protein Interactions: RBBP7; RBBP4; HDAC2; HDAC1; CHD4; MTA2; MTA1; POLR2H; POLR2C; POLR2A; DNMT1; LEO1; CDC73; PAF1; RTF1; CTR9; NRIP1; EZH2; DNMT3L; SUZ12; SIRT1; EED; UBC; EPHB1; CREB1; CBX5; TRIM28; ZFP57; UHRF2; UHRF1; MAP1LC3B; UBE2W; PLEKHJ1; DUSP23; CMTM6; PAM16; TSC22