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DNMT1 Antibody (OALA02447)

Catalog#: OALA02447
Domestic: within 1 week delivery International: 1 week
This product is Genway GWB-CB814A
More Information
Predicted Species Reactivity Cow, Dog, Pig, Sheep
Product Format Liquid
Clonality Monoclonal
Clone 60B1220.1
Isotype IgG1
Host Mouse
Application IHC-P, WB, IP, CHIP
Reconstitution and Storage Long term: -20C; Short term: +4C. Avoid repeat freeze-thaw cycles.
Replacement Item This antibody may replace item sc-10219 from Santa Cruz Biotechnology.
Immunogen DNMT / DNMT1 antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bat, Horse, Rabbit (100%); Sheep, Hamster, Elephant, Dog, Bovine, Pig (93%).
Purification Protein G purified
Predicted Homology Based on Immunogen Sequence Cow; Dog; Pig; Sheep: 90%
Concentration 0.5 mg/ml
Datasheets/Manuals Printable datasheet for OALA02447
Specificity Dnmt1 (Genbank Accession No. NP_001370). It will cross react with Dnmt1.
Epitope aa637-650
Warning For research use only.
Application Info IHC - Paraffin (5 ug/ml), Western blot (2 - 4 ug/ml), Immunoprecipitation, Chromatin Immunoprecipitation
Application Data Immunohistochemistry: OALA02447 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA02447 was determined to be 5 ug/ml.
Storage Buffer PBS, 0.05% BSA, 0.05% sodium azide.
Gene Symbol DNMT1
Alias Symbols DNMT1, AIM, CXXC finger protein 9, CXXC9, DNA methyltransferase 1, DNMT, DNA methyltransferase HsaI, DNA MTase HsaI, HSN1E, M.HsaI, MCMT
NCBI Gene Id 1786
Swissprot Id P26358
Protein Accession # NP_001370.1
Nucleotide Accession # NM_001379
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express DNMT1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express DNMT1.
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