DNMT1 Antibody : Biotin (OASE00145-Biotin)

Catalog No: OASE00145-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 60B1220.1
• Isotype: IgG1 Kappa
• Host: Mouse
• Conjugation: Biotin
• Application: WB, IHC, IP, CHIP
• Additional Information: Background Info: It will cross-react with mouse DNMT1.
  Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation
  of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family
  members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
  Certificate of Analysis: 2 ug/mL was sufficient for detection of Dnmt1 in 10ug of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1 (Gene accession number NP_001370)
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 2-4ug/mL (WB), 1-2ug/mL (IHC)
• Storage Buffer: PBS, 0.05% BSA
• Reference: 1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl.
  Acids Res. 20: 2287-2291.
  3. Xie S. et al. (1999) Gene 236: 87-95.
  4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99:
  247-257.
  5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382.
  6. Robertson K.D., et al. (2000) Nat Genet 25(3):338-342.

Target Info

• Gene Symbol: DNMT1
• Gene Full Name: DNA (cytosine-5-)-methyltransferase 1
• Alias Symbols: AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN, m.HsaI
• NCBI Gene Id: 1786
• Uniprot ID: P26358
• Protein Accession #: NP_001370