Catalog No: OKEH06943
Size:96T
Price: $525.00
SKU
OKEH06943
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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DNM2 ELISA Kit (Mouse) (OKEH06943)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH06943
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Dnm2 ELISA Kit (Mouse) (OKEH06943) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dnm2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dnm2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dnm2 captured in the well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into matrix = 92.1%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <4.6%
Inter-Assay CV <7.6%
ELISA Kit Component
ComponentAmount
Dnm2 Microplate96 Wells (12 x 8 Well strips)
Dnm2 Lyophilized Standard2 x 1 ng
100X Dnm2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity7.92 pg/mL
SpecificityNatural and recombinant Mouse Dynamin-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDnm2
Gene Full Namedynamin 2
Alias Symbolsb2b2159C;b2b2159Clo;Dyn;Dyn2;Dynamin UDNM;dynamin-2;Udnm.
NCBI Gene Id13430
Protein NameP39054
Description of TargetDynamin-2
Uniprot IDhttps://www.uniprot.org/uniprot/P39054
Protein Accession #NP_001240822
Nucleotide Accession #NM_001253893
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