Catalog No: OKEH03509
Size:96 Wells
Price: $525.00
SKU
OKEH03509
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DNASE1L3 ELISA Kit (Mouse) (OKEH03509) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dnase1l3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Dnase1l3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dnase1l3 captured in well.
ELISA Kit Range0.156 - 10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.2% (n = 20)
Mean Inter-assay CV%: <= 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Dnase1l3 Microplate96 Wells (12 x 8 Well strips)
Dnase1l3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Dnase1l3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Mouse Deoxyribonuclease gamma
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDNASE1L3
Gene Full Namedeoxyribonuclease 1-like 3
Alias SymbolsLsd, Dhp2, DNase, DNasegamma
NCBI Gene Id13421
Protein NameDeoxyribonuclease gamma
Description of TargetHas DNA hydrolytic activity. Does not bind to actin.
Swissprot IdO55070
Protein Accession #NP_031896.3
Nucleotide Accession #NM_007870.3
Protein Size (# AA)310
Molecular Weight36 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Dnase1l3.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Dnase1l3.
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