DNASE1L3 Antibody (OABF01811)

Catalog No: OABF01811

Size:100UL

Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti DNase gamma Polyclonal Antibody, Unconjugated (OABF01811) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti DNase gamma Polyclonal Antibody, Unconjugated (OABF01811) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded mouse liver labeled with Anti-DNase gamma Polyclonal Antibody, Unconjugated (OABF00011) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37C.

Product Info

• Predicted Species Reactivity: Horse|Human|Mouse|Porcine|Rabbit|Rat
• Product Format: Liquid. Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Subcellular location: Nucleus
• Reconstitution and Storage: -20°C
• Immunogen: KLH conjugated synthetic peptide derived from human DNase gamma
• Purification: Protein A Purified
• Concentration: 1 ug/ul
• Application Info: WB: 1:300 - 1,000
  IHC-P: 1:200 - 400
  Fluorescent (IHC-P): 1:50 - 200
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: DNASE1L3
• Gene Full Name: deoxyribonuclease 1 like 3
• Alias Symbols: deoxyribonuclease gamma;deoxyribonuclease I-like 3;deoxyribonuclease I-like III;DHP2;DNAS1L3;DNase gamma;DNase I homolog protein 2;DNase I homolog protein DHP2;DNase I-like 3;Liver and spleen DNase;LSD;LS-DNase;SLEB16.
• NCBI Gene Id: 1776
• Protein Name: Deoxyribonuclease gamma
• Description of Target: Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed:9070308, PubMed:9714828, PubMed:14646506, PubMed:10807908, PubMed:27293190). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed:23229555, PubMed:24312463). The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed:27293190). Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
• Uniprot ID: Q13609