DNASE1 ELISA Kit (Pig) (OKEH03902)

Catalog No: OKEH03902

Size:96 Wells

Pig DNASE1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Pig
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology DNASE1 ELISA Kit (Pig) (OKEH03902) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNASE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNASE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNASE1 captured in well.
• ELISA Kit Range: 31.2 - 2000pg/ml
• ELISA Kit Reproducibility: Mean Intra-Assay Precision - < 4.4%, n = 20
  Mean Inter-Assay Precision - < 7.3%, n = 20
• ELISA Kit Component:
  • Anti-DNASE1 Microplate: 96 Wells (12 x 8 Well strips)
  • DNASE1 Lyophilized Standard: 2
  • 100X Biotinylated DNASE1 Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 7.6 pg/mL
• Specificity: Natural and recombinant Pig Deoxyribonuclease-1
• Publications:

  Degraded neutrophil extracellular traps promote the growth of Actinobacillus pleuropneumoniae. Cell Death Dis. 10, 657 (2019). 31506432

  Role of Bacterial and Host DNases on Host-Pathogen Interaction during Streptococcus suis Meningitis. Int J Mol Sci. 21, NULL (2020). 32722502

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DNASE1
• Gene Full Name: deoxyribonuclease I
• Alias Symbols: Deoxyribonuclease-1; Deoxyribonuclease I; DNL1
• NCBI Gene Id: 397051
• Protein Name: Deoxyribonuclease-1
• Description of Target: Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
• Uniprot ID: P11936
• Protein Accession #: NP_999156.1
• Nucleotide Accession #: NM_213991.1
• Protein Size (# AA): 284
• Molecular Weight: 32 kDa