Catalog No: OKEH03902
Price: $675.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DNASE1 ELISA Kit (Pig) (OKEH03902) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNASE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNASE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNASE1 captured in well.
ELISA Kit Range31.2-2000pg/ml
ELISA Kit ReproducibilityMean Intra-Assay Precision - < 4.4%, n = 20
Mean Inter-Assay Precision - < 7.3%, n = 20
ELISA Kit Component
Anti-DNASE1 Microplate96 Wells (12 x 8 Well strips)
DNASE1 Lyophilized Standard2
100X Biotinylated DNASE1 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.6 pg/mL
SpecificityNatural and recombinant Pig Deoxyribonuclease-1

Degraded neutrophil extracellular traps promote the growth of Actinobacillus pleuropneumoniae. Cell Death Dis. 10, 657 (2019). 31506432

Role of Bacterial and Host DNases on Host-Pathogen Interaction during Streptococcus suis Meningitis. Int J Mol Sci. 21, NULL (2020). 32722502

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDNASE1
Gene Full Namedeoxyribonuclease 1
Alias Symbolsdeoxyribonuclease I;deoxyribonuclease-1;DNase I.
NCBI Gene Id397051
Protein NameP11936
Description of TargetDeoxyribonuclease-1
Uniprot IDhttps://www.uniprot.org/uniprot/P11936
Protein Accession #NP_999156.1
Nucleotide Accession #NM_213991.1
Protein Size (# AA)284
Molecular Weight32 kDa
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