DNAJC6 Antibody - middle region (OAAB06583)

Catalog No: OAAB06583

Size:400UL

DNAJC6 Antibody (Center) (Cat. #OAAB06583) western blot analysis in K562(lane 1), HL-60 cell line(lane 2) and mouse brain tissue(lane 3) lysates (35ug/lane).This demonstrates the DNAJC6 antibody detected the DNAJC6 protein (arrow).

DNAJC6 Antibody (Center) (Cat. #OAAB06583) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNAJC6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

DNAJC6 Antibody (Center) (Cat. #OAAB06583) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DNAJC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-281 amino acids from the Central region of human DNAJC6.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  DNAJC6 is supported by BioGPS gene expression data to be expressed in HL60

  DNAJC6 is strongly supported by BioGPS gene expression data to be expressed in K562

• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: DNAJC6 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: DNAJC6
• Alias Symbols: 2810027M23Rik, au, auxilin, DJC6, DnaJ (Hsp40) homolog, subfamily B, member 6, DnaJ (Hsp40) homolog, subfamily C, member 6, dnaJ homolog subfamily C member 6, mKIAA0473, PARK19, putative tyrosine-protein phosphatase auxilin.
• NCBI Gene Id: 72685|9829
• Protein Name: Putative tyrosine-protein phosphatase auxilin|putative tyrosine-protein phosphatase auxilin isoform 1 [Homo sapiens]|putative tyrosine-protein phosphatase auxilin isoform 2 [Homo sapiens]|putative tyrosine-protein phosphatase auxilin isoform 3 [Homo sapiens]
• Description of Target: Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons (By similarity).|Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons.
• Uniprot ID: O75061|Q80TZ3
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001243793.1,NP_001243794.1,NP_055602.1
• Molecular Weight: 99997