Catalog No: OKCD02114 (Formerly GWB-KBBUH3)
Size:96WELLS
Price: $750.00
SKU
OKCD02114
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Datasheets/ManualsPrintable datasheet for DNAJC13 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)84-102%90-97%81-91%84-91%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum79-9487
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DNAJC13 Microplate96 Wells (12 x 8 Well Strips)
DNAJC13 Lyophilized Standard2 x 50 ng
100X Biotinylated DNAJC13 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.33 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13). No significant cross-reactivity or interference between DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDNAJC13
Gene Full NameDnaJ heat shock protein family (Hsp40) member C13
Alias SymbolsRME8, PARK21
NCBI Gene Id23317
Protein NameDnaJ homolog subfamily C member 13
Description of TargetInvolved in membrane trafficking through early endosomes, such as the early endosome to recycling endosome transport implicated in the recycling of transferrin and the early endosome to late endosome transport implicated in degradation of EGF and EGFR.
Uniprot IDO75165
Protein Accession #NP_056083.3
Nucleotide Accession #NM_015268.3
Protein Size (# AA)2243
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