DNAJC12 ELISA Kit (Human) (OKCD00592)

Catalog No: OKCD00592 (Formerly GWB-KBBTX8)

Size:96WELLS

Human DNAJC12 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DNAJC12 Microplate: 96 Wells (12 x 8 Well Strips)
  • DNAJC12 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated DNAJC12 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12). No significant cross-reactivity or interference between DnaJ/HSP40 Homolog Subfamily C, Member 12 (DNAJC12) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DNAJC12
• Gene Full Name: DnaJ heat shock protein family (Hsp40) member C12
• Alias Symbols: DnaJ (Hsp40) homolog, subfamily C, member 12;dnaJ homolog subfamily C member 12;HPANBH4;J domain containing protein 1 (JDP1);J domain protein 1;j domain-containing protein 1;JDP1.
• NCBI Gene Id: 56521
• Protein Name: DnaJ homolog subfamily C member 12
• Description of Target: DnaJ homolog subfamily C member 12
• Uniprot ID: Q9UKB3
• Protein Accession #: NP_068572
• Nucleotide Accession #: NM_021800.2
• Protein Size (# AA): 198