Catalog No: OKEI00258
Size:96 Wells
Price: $525.00
SKU
OKEI00258
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DNAJB2 ELISA Kit (Human) (OKEI00258) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNAJB2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNAJB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample DNAJB2
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level DNAJB2. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level DNAJB2 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
DNAJB2 Microplate96 Wells (12 x 8 Well strips)
DNAJB2 Lyophilized Standard2
100X DNAJB2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
30X Wash Buffer1 x 30 mL
100X Luminol Substrate1 x 10 mL
Substrate Diluent1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Biological Fluids
Sensitivity0.094 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDNAJB2
Alias SymbolsHSJ1, CMT2T, DSMA5, HSJ-1, HSPF3
NCBI Gene Id3300
Protein NameDnaJ homolog subfamily B member 2
Description of TargetFunctions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family . In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins . Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes . Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins .
Uniprot IDP25686
Protein Accession #NP_001034639
Nucleotide Accession #NM_001039550
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