Catalog No: OPED00066
Size:50 ug
Price: $252.00
SKU
OPED00066
Availability: Domestic: within 1-2 week delivery | International: 1-2 week
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Datasheets/Manuals | Printable datasheet for DNAJB1 Recombinant Protein (Human) (OPED00066) (OPED00066) |
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Predicted Species Reactivity | Homo sapiens|Human |
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Product Format | Liquid. In Dulbecco’s PBS. |
Host | E. Coli |
Application | WB, EMSA |
Additional Information | Hsp40/Hdj1 is a cytosolic co-chaperone belonging to the class DnaJ, named after its homolog in E. Coli. Hsp40 functions in protein folding by binding nascent peptides and unfolded substrates and facilitating substrate interaction with Hsp70. Binding of Hsp40 to Hsp70 increases Hsp70 ATP hydrolysis and substrate binding, which is reversed by the action of nucleotide exchange factors (GrpE in E. Coli). Repeated cycles of Hsp40/Hsp70 peptide binding and ATP hydrolysis prevent premature folding and aggregation, promoting protein maturation throughout the cell. |
Reconstitution and Storage | Store at -80C |
Purification | Purified by multi-step chromatography. |
Purity | >80% (SDS-PAGE) |
Application Info | Western blot control. |
Reference | Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries: N.H. Benbahouche, et al.; J. Biol. Chem. 289, 6236 (2014), Application(s): Cell Culture, Abstract; Full Text Biochemical characterization of the apicoplast-targeted AAA+ ATPase ClpB from Plasmodium falciparum: F. Ngansop, et al.; Biochem. Biophys. Res. Commun. 439, 191 (2013), Application(s): Luminescence Assay, Abstract; Purification of hsp104, a protein disaggregase: E.A. Sweeny, et al.; J. Vis. Exp. 55, e3190 (2011), Application(s): Luminescence Assay, Abstract; Full Text Antigen microarrays identify unique serum autoantibody signatures inclinical and pathologic subtypes of multiple sclerosis: H. Weiner, et al. ; PNAS 105, 18889 (2008), Application(s): Antigen Microarray , Abstract; 3'Sulfogalactolipid binding specifically inhibits HSP70 ATPase activity in vitro: C. Lingwood, et al. ; Biochemistry 42, 1611 (2003), Application(s): In Vitro Assay , Abstract; Activation of the herpes simplex virus type-1 origin-binding protein (UL9) by heat shock proteins: P.E. Boehmer, et al. ; J. Biol. Chem. 277, 5660 (2002), Application(s): EMSA, In Vitro Assay , Abstract; Control of PERK eIF2alpha kinase activity by the endoplasmic reticulum stress-induced molecular chaperone P58IPK: M.G. Katze, et al. ; PNAS 99, 15920 (2002), Application(s): In Vitro Assay , Abstract; alpha -Crystallin prevents irreversible protein denaturation and acts cooperatively with other heat-shock proteins to renature the stabilized partially denatured protein in an ATP-dependent manner: A. Spector, et al. ; Eur. J. Biochem. 267, 4705 (2000), Application(s): In Vitro Assay , Abstract; |
Gene Symbol | DNAJB1 |
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Gene Full Name | DnaJ heat shock protein family (Hsp40) member B1 |
Alias Symbols | DnaJ (Hsp40) homolog, subfamily B, member 1;dnaJ homolog subfamily B member 1;dnaJ protein homolog 1;Hdj1;heat shock 40 kDa protein 1;Hsp40;HSPF1;human DnaJ protein 1;radial spoke 16 homolog B;RSPH16B;Sis1. |
NCBI Gene Id | 3337 |
Protein Name | DnaJ homolog subfamily B member 1 |
Description of Target | Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877). |
Uniprot ID | P25685 |
Protein Accession # | NP_001129064.1 |
Nucleotide Accession # | NM_001135592.2 |
Protein Size (# AA) | Recombinant |
Molecular Weight | 40 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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