DNAJB1 Antibody (OAAF01834)

Catalog No: OAAF01834 (Formerly GWB-ASD105)

Size:100 ug

Western blot analysis of extracts from COLO205 cells, using HSP40 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HSP40 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using HSP40 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human HSP40.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: HSP40 antibody detects endogenous levels of total HSP40 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:300~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000
• Reference: M. Patricia Hernández, J. Biol. Chem., Mar 2002; 277: 11873 - 11881.
  Annemieke A. Michels, J. Biol. Chem., Dec 1997; 272: 33283.
  Fritha Hennessy, Protein Sci., Jul 2005; 14: 1697 - 1709.
  Mark W. Melville, PNAS, Jan 1997; 94: 97.

Target Info

• Gene Symbol: DNAJB1
• Gene Full Name: DnaJ heat shock protein family (Hsp40) member B1
• Alias Symbols: DnaJ (Hsp40) homolog, subfamily B, member 1;dnaJ homolog subfamily B member 1;dnaJ protein homolog 1;Hdj1;heat shock 40 kDa protein 1;Hsp40;HSPF1;human DnaJ protein 1;radial spoke 16 homolog B;RSPH16B;Sis1.
• NCBI Gene Id: 3337
• Protein Name: DnaJ homolog subfamily B member 1
• Description of Target: Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).
• Uniprot ID: P25685
• Protein Accession #: NP_006136.1
• Molecular Weight: 38 kDa