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Catalog No: OKEH07968
Size:96T
Price: $725.00
SKU
OKEH07968
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH07968
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology DNAI1 ELISA Kit (Human) (OKEH07968) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNAI1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNAI1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNAI1 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.6% (n = 20)
Mean Inter-assay CV%: <=11.6% (n = 20)
ELISA Kit Component
ComponentAmount
DNAI1 Microplate96 Wells (12 x 8 Well strips)
DNAI1 Lyophilized Standard2
100X Biotinylated DNAI1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Part of the dynein complex of respiratory cilia.
::Subcellular Location: Cytoplasm Cytoskeleton Cilium axoneme
::Unigene: Hs.112667
String: 9606.ENSP00000242317
::KEGG: hsa:27019
MIM: 244400
Pfam: PF00400
Reconstitution and StorageStore as indicated in product manual.
Sensitivity9.5pg/mL
SpecificityNatural and recombinant Human Dynein intermediate chain 1, axonemal
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDNAI1
Gene Full Namedynein axonemal intermediate chain 1
Alias SymbolsAxonemal dynein intermediate chain 1;CILD1;DIC1;dynein axonemal intermediate chain 1;dynein, axonemal, intermediate polypeptide 1;ICS1;immotile cilia syndrome 1;PCD;testis tissue sperm-binding protein Li 87P.
NCBI Gene Id27019
Protein NameQ9UI46
Description of TargetDynein intermediate chain 1, axonemal
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UI46
Protein Accession #NP_001268357.1
Nucleotide Accession #NM_001281428.1
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