Catalog No: OKEH07968
Size:96T
Price: $725.00
SKU
OKEH07968
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology DNAI1 ELISA Kit (Human) (OKEH07968) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNAI1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNAI1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNAI1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 31.2-2000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=5.6% (n = 20) Mean Inter-assay CV%: <=11.6% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Part of the dynein complex of respiratory cilia. | ||||||||||||||||||||||
:: | Subcellular Location: Cytoplasm Cytoskeleton Cilium axoneme | ||||||||||||||||||||||
:: | Unigene: Hs.112667 String: 9606.ENSP00000242317 | ||||||||||||||||||||||
:: | KEGG: hsa:27019 MIM: 244400 Pfam: PF00400 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 9.5pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Dynein intermediate chain 1, axonemal | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | DNAI1 |
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Gene Full Name | dynein axonemal intermediate chain 1 |
Alias Symbols | Axonemal dynein intermediate chain 1;CILD1;DIC1;dynein axonemal intermediate chain 1;dynein, axonemal, intermediate polypeptide 1;ICS1;immotile cilia syndrome 1;PCD;testis tissue sperm-binding protein Li 87P. |
NCBI Gene Id | 27019 |
Protein Name | Q9UI46 |
Description of Target | Dynein intermediate chain 1, axonemal |
Uniprot ID | https://www.uniprot.org/uniprot/Q9UI46 |
Protein Accession # | NP_001268357.1 |
Nucleotide Accession # | NM_001281428.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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