DNAH17 ELISA Kit (Mouse) (OKEH05070)

Catalog No: OKEH05070

Size:96 Wells

Mouse DNAH17 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Dnah17 ELISA Kit (Mouse) (OKEH05070) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dnah17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dnah17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dnah17 captured in the well.
• ELISA Kit Range: 0.156 - 10ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 92.7%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <5.2%, n = 20
  Mean Inter-Assay Precision - <7.3%, n = 20
• ELISA Kit Component:
  • DNAH17 Microplate: 96 Wells (12 x 8 Well strips)
  • DNAH17 Lyophilized Standard: 2
  • 100X Biotinylated DNAH17 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.084 ng/mL
• Specificity: Natural and recombinant Mouse Dynein heavy chain 17, axonemal
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DNAH17
• Gene Full Name: Dynein heavy chain 17, axonemal
• Alias Symbols: Dnah, Dnahc, Gm1178, Dnahc17, Dnahcl1, mKIAA3028, 2810003K23Rik
• NCBI Gene Id: 69926
• Protein Name: Dynein heavy chain 17, axonemal
• Description of Target: Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly.
• Uniprot ID: Q69Z23
• Protein Accession #: NP_001161218.1
• Nucleotide Accession #: NM_001167746.1
• Protein Size (# AA): 4481