DNAH17 ELISA Kit (Human) (OKEH01776)

Catalog No: OKEH01776

Size:96T

Human DNAH17 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology DNAH17 ELISA Kit (Human) (OKEH01776) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human DNAH17 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DNAH17 and incubated. The DNAH17 found in the sample or standards competes with the biotinylated DNAH17 for limited binding sites on the immobilized anti-human DNAH17 antibody. Excess unbound biotinylated DNAH17 and sample or standard DNAH17 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DNAH17 captured in the well and inversely proportional to the amount of DNAH17 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.6% (n = 20)
  Mean Inter-assay CV%: < 9.6% (n = 20)
• ELISA Kit Component:
  • Anti-DNAH17 Microplate: 96 Wells (12 x 8 Well strips)
  • DNAH17 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X DNAH17-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.12 ng/mL
• Specificity: Natural and recombinant Human Dynein heavy chain 17, axonemal
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: DNAH17
• Gene Full Name: dynein axonemal heavy chain 17
• Alias Symbols: Axonemal beta dynein heavy chain 17;Axonemal dynein heavy chain-like protein 1;Ciliary dynein heavy chain 17;Ciliary dynein heavy chain-like protein 1;Dynein light chain 2, axonemal.
• NCBI Gene Id: 8632
• Protein Name: Q9UFH2
• Description of Target: Dynein heavy chain 17, axonemal
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UFH2
• Protein Accession #: NP_775899.3
• Nucleotide Accession #: NM_173628.3
• Protein Size (# AA): 4485
• Molecular Weight: 511 kDa