DNAH11 ELISA Kit (Human) (OKCD01051)

Catalog No: OKCD01051 (Formerly GWB-KBBGZ5)

Size:96WELLS

Human DNAH11 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Axonemal, Heavy Chain 11 (DNAH11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynein, Axonemal, Heavy Chain 11 (DNAH11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein, Axonemal, Heavy Chain 11 (DNAH11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dynein, Axonemal, Heavy Chain 11 (DNAH11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dynein, Axonemal, Heavy Chain 11 (DNAH11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dynein, Axonemal, Heavy Chain 11 (DNAH11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DNAH11 Microplate: 96 Wells (12 x 8 Well Strips)
  • DNAH11 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated DNAH11 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.127 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dynein, Axonemal, Heavy Chain 11 (DNAH11). No significant cross-reactivity or interference between Dynein, Axonemal, Heavy Chain 11 (DNAH11) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DNAH11
• Gene Full Name: dynein axonemal heavy chain 11
• Alias Symbols: axonemal beta dynein heavy chain 11;axonemal dynein heavy chain 11;CILD7;ciliary dynein heavy chain 11;DNAHBL;DNAHC11;DNHBL;DPL11;dynein axonemal heavy chain 11;dynein heavy chain 11, axonemal;dynein, axonemal, heavy polypeptide 11;dynein, ciliary, heavy chain 11.
• NCBI Gene Id: 8701
• Protein Name: Dynein heavy chain 11, axonemal
• Description of Target: Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
• Uniprot ID: Q96DT5
• Protein Accession #: NP_001264044
• Nucleotide Accession #: NM_001277115.1
• Protein Size (# AA): 4516