DNA/RNA Damage Antibody : Biotin (OASE00081-Biotin)

Catalog No: OASE00081-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 15A3
• Isotype: IgG2b
• Host: Mouse
• Conjugation: Biotin
• Application: ELISA, IHC, ICC, Immunoaffinity columns
• Additional Information: Background Info: Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
  Scientific Background: DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then
  hinder the ability of the cell to carry out its functions.
  There are four main types of DNA due to endogenous
  cellular processes and they are oxidation, alkylation,
  hydrolysis and mismatch of the bases. During the
  oxidation of bases, highly reactive chemical entities
  collectively known as RONS, occurs. RONS stands for
  reactive oxygen and nitrogen species and includes nitric
  oxide, superoxide, hydroxyl radical, hydrogen peroxide
  and peroxynitrite. Numerous studies have shown that
  RONS causes a variety of issues including DNA damage
  (1).
  8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8-
  hydroxyguanosine are all RNA and DNA markers of
  oxidative damage. 8-hydroxy-2'-guanosine is produced
  by reactive oxygen and nitrogen species including
  hydroxyl radical and peroxynitrite. Specifically its high
  biological relevance is due to its ability to induce G to T
  transversions, which is one of the most frequent somatic
  mutations (2). 8-hydroxy-guanine has been the most
  frequently studied type of DNA base damage, with
  studies in diabetes, and cancer. Base modifications of
  this type arise from radical-induced hydroxylation and
  cleavage reactions of the purine ring (3, 4). And finally,
  8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine,
  induces a mutagenic transversion of G to T in DNA. Its
  role has specifically been tested in the development of
  diabetes, hypertension and strokes (5, 6, and 7).
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: 8-hydroxy-guanosine-BSA and –casein conjugates
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Storage Buffer: PBS, 50% glycerol
• Reference: 1. Kim H.W., Murakami A., Williams M.V., and Ohigashi H. (2003) Carcinogenesis 24(2): 235-241.
  2. Pilger A. and Rudiger H.W. (2006) Int Arch Occup
  Environ Health. 80(1): 1-15.
  3. Malins D.C. and Haimanot R. (1991) Cancer Res.
  51(19): 5430-5432.
  4. Kvam E. and Tyrrell R.M. (1997) Carcinogenesis 18(11):
  2281-2283.
  5. Kowluru R.A., Atasi L., and Ho Y.S. (2006) Invest
  Ophthalmol Vis Sci 47(4): 1594-9.
  6. Bowers R. et al. (2004) Am J Respir Crit Care Med.
  169(6): 764-9.
  7. Cui J., Holmes E.H., Greene T.G., and Liu P.K. (2000)
  Faseb J. 14(7): 955-67.