DMPK ELISA Kit (Mouse) (OKEH08194)

Catalog No: OKEH08194

Size:96T

Mouse DMPK ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	104-114% (n = 20)	99-108% (n = 20)	90-102% (n = 20)	103-111% (n = 20)
  EDTA plasma(n=5)	92-102% (n = 20)	100-108% (n = 20)	99-108% (n = 20)	103-112% (n = 20)
  heparin plasma(n=5)	89-97% (n = 20)	97-107% (n = 20)	101-111% (n = 20)	87-97% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology DMPK ELISA Kit (OKEH08194) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-DMPK antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DMPK and incubated. The DMPK found in the sample or standards competes with the biotinylated DMPK for limited binding sites on the immobilized anti-DMPK antibody. Excess unbound biotinylated DMPK and sample or standard DMPK is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DMPK captured in the well and inversely proportional to the amount of DMPK which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.4% (n = 20)
  Mean Inter-assay CV%: <=7.6% (n = 20)
• ELISA Kit Component:
  • Anti-DMPK Microplate: 96 Wells (12 x 8 Well strips)
  • DMPK Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X DMPK-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity.
  Subcellular Location: Isoform 5 Cytoplasm Cytosol
  Unigene: Mm.6529
  SMR: P54265
  String: 10090.ENSMUSP00000032568
  KEGG: mmu:13400
  Pfam: PF08826
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.091ng/mL
• Specificity: Natural and recombinant Mouse Myotonin-protein kinase
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Dmpk
• Gene Full Name: dystrophia myotonica-protein kinase
• Alias Symbols: DM;Dm15;DMK;DM-kinase;DMPK;dystrophia myotonica kinase, B15;MDPK;MT-PK;myotonic dystrophy protein kinase;myotonin-protein kinase.
• NCBI Gene Id: 13400
• Protein Name: P54265
• Description of Target: Myotonin-protein kinase
• Uniprot ID: https://www.uniprot.org/uniprot/P54265
• Protein Accession #: NP_001177420.1
• Nucleotide Accession #: NM_001190491.1