DMPK ELISA Kit (Human) (OKEH08193)

Catalog No: OKEH08193

Size:96T

Human DMPK ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology DMPK ELISA Kit (Human) (OKEH08193) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DMPK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DMPK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DMPK captured in well.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.0% (n = 20)
  Mean Inter-assay CV%: <=7.5% (n = 20)
• ELISA Kit Component:
  • DMPK Microplate: 96 Wells (12 x 8 Well strips)
  • DMPK Lyophilized Standard: 2
  • 100X Biotinylated DMPK Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity.
  Subcellular Location: Isoform 3 Mitochondrion membrane
  Unigene: Hs.631596
  SMR: Q09013
  String: 9606.ENSP00000345997
  KEGG: hsa:1760
  MIM: 160900
  Pfam: PF08826
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Human Myotonin-protein kinase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DMPK
• Gene Full Name: DM1 protein kinase
• Alias Symbols: DM;DM protein kinase;DM1;DM1 protein kinase;DM1PK;DMK;DM-kinase;DMPK;dystrophia myotonica protein kinase;MDPK;MT-PK;myotonic dystrophy associated protein kinase;Myotonic dystrophy protein kinase;myotonin protein kinase A;myotonin-protein kinase;thymopoietin homolog.
• NCBI Gene Id: 1760
• Protein Name: Q09013
• Description of Target: Myotonin-protein kinase
• Uniprot ID: https://www.uniprot.org/uniprot/Q09013
• Protein Accession #: NP_001075029.1
• Nucleotide Accession #: NM_001081560.2