DMBT1 ELISA Kit (Pig) (OKEH08140)

Catalog No: OKEH08140

Size:96T

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	89-100% (n = 20)	104-114% (n = 20)	104-113% (n = 20)	103-113% (n = 20)
  EDTA plasma(n=5)	94-104% (n = 20)	87-96% (n = 20)	96-106% (n = 20)	109-118% (n = 20)
  heparin plasma(n=5)	88-97% (n = 20)	98-108% (n = 20)	111-121% (n = 20)	101-112% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology DMBT1 ELISA Kit (Pig) (OKEH08140) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DMBT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DMBT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DMBT1 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.9% (n = 20)
  Mean Inter-assay CV%: <=9.6% (n = 20)
• ELISA Kit Component:
  • DMBT1 Microplate: 96 Wells (12 x 8 Well strips)
  • DMBT1 Lyophilized Standard: 2
  • 100X Biotinylated DMBT1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May play roles in mucosal defense system and cellular immune defense. May play a role in liver regeneration. May be an important factor in fate decision and differentiation of transit-amplifying ductular (oval) cells within the hepatic lineage. May function as a binding protein in saliva for the regulation of taste sensation. May play a role as an opsonin receptor for SFTPD and SPAR in macrophage tissues throughout the body, including epithelial cells lining the gastrointestinal tract. Required for terminal differentiation of columnar epithelial cells during early embryogenesis. Displays a broad calcium-dependent binding spectrum against both Gram-positive and Gram-negative bacteria, suggesting a role in defense against bacterial pathogens. Binds to a range of poly-sulfated and poly-phosphorylated ligands which may explain its broad bacterial-binding specificity. Inhibits cytoinvasion of S.enterica. Associates with the actin cytoskeleton and is involved in its remodeling during regulated exocytosis. Interacts with pancreatic zymogens in a pH-dependent manner and may act as a Golgi cargo receptor in the regulated secretory pathway of the pancreatic acinar cell.
  Subcellular Location: Secreted Cytoplasmic vesicle Secretory vesicle membrane Single-pass membrane protein Lumenal side Localized to the lumenal aspect of crypt cells in the small intestine. In the colon, seen in the lumenal aspect of surface epithelial cells (By similarity). Formed in the ducts of von Ebner gland and released into the fluid bathing the taste buds contained in the taste papillae (By similarity).
  Unigene: Ssc.8694
  KEGG: ssc:751862
  Pfam: PF00431
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Pig Deleted in malignant brain tumors 1 protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DMBT1
• Gene Full Name: deleted in malignant brain tumors 1
• Alias Symbols: deleted in malignant brain tumors 1 protein;hensin.
• NCBI Gene Id: 751862
• Protein Name: Q4A3R3
• Description of Target: Deleted in malignant brain tumors 1 protein
• Uniprot ID: https://www.uniprot.org/uniprot/Q4A3R3
• Protein Accession #: NP_001041653.1
• Nucleotide Accession #: NM_001048188.1