DLL4 ELISA Kit (Mouse) (OKBB00576)

Catalog No: OKBB00576

Size:96 Wells

Mouse DLL4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse DLL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DLL4 has been precoated onto 96-well plates. Standards(NS0, S28-P525) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DLL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse DLL4 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	2.32	4.2	6.12	2.41	3.68	7.35
  SD	0.09	0.14	0.26	0.19	0.3	0.54
  CV%	3.9	3.3	4.2	7.9	8.2	7.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse DLL4 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse DLL4 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse DLL4 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse DLL4
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S28-P525
• Description: For quantitative detection of mouse DLL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: DLL4
• Gene Full Name: delta-like 4 (Drosophila)
• Alias Symbols: Delta 4, Delta4, Delta ligand 4, Delta like 4, DLL 4, DLL4, hdelta2, Homeobox protein DLL-4, Notch ligand delta 2, Notch ligand DLL4, XDLL-4, Q9NR61
• NCBI Gene Id: 54485
• Protein Name: Delta-like protein 4
• Description of Target: Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. Mouse DLL4 could activate mouse Notch1 and mouse Notch4. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.
• Uniprot ID: Q9JI71
• Protein Accession #: NP_062327.2
• Nucleotide Accession #: NM_019454.3