Catalog No: OKDD00231 (Formerly GWB-KBBRK5)
Size:96 Wells
Price: $800.00
SKU
OKDD00231
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Datasheets/ManualsPrintable datasheet for dLL3 ELISA Kit (Human) (OKDD00231)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology dLL3 ELISA Kit (Human) (OKDD00231) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for dLL3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for dLL3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample dLL3 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DLL3 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DLL3 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-dLL3 Microplate96 Wells (12 x 8 Well Strips)
dLL3 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X dLL3 Biotinylated Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Detection Conjugate1 x 120 ÂuL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates
Sensitivity< 0.069 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of DLL3. No significant cross-reactivity or interference between DLL3 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolDLL3
Gene Full Namedelta like canonical Notch ligand 3
Alias Symbolsdelta3;delta-like 3;delta-like protein 3;drosophila Delta homolog 3;SCDO1.
NCBI Gene Id10683
Protein NameDelta-like protein 3
Description of TargetInhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
Uniprot IDQ9NYJ7
Protein Accession #NP_058637.1
Nucleotide Accession #NM_016941.3
Protein Size (# AA)618
Molecular Weight65 kDa
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