Catalog No: OKCD02500
Size:96WELLS
Price: $750.00
SKU
OKCD02500
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Datasheets/ManualsPrintable datasheet for DLK1 ELISA Kit (Mouse) (OKCD02500)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-101%90-101%89-97%80-104%
EDTA Plasma (n=5)79-92%82-102%78-103%83-97%
Heparin Plasma (n=5)87-99%90-101%80-93%88-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Delta Like 1 Homolog (dLK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Delta Like 1 Homolog (dLK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Delta Like 1 Homolog (dLK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Delta Like 1 Homolog (dLK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)95-104101
EDTA Plasma (n=5)83-9590
Heparin Plasma(n=5)86-9490
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Delta Like 1 Homolog (dLK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Delta Like 1 Homolog (dLK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DLK1 Microplate96 Wells (12 x 8 Well strips)
DLK1 Lyophilized Standard2
100X Biotinylated DLK1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, urine and other biological fluids
Sensitivity0.67 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Delta Like 1 Homolog (dLK1). No significant cross-reactivity or interference between Delta Like 1 Homolog (dLK1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolDlk1
Gene Full Namedelta like non-canonical Notch ligand 1
Alias Symbolsadipocyte differentiation inhibitor protein;AW742678;delta-like 1 homolog;Dl;DLK-1;DlkI;FA;FA1;fetal antigen 1;Ly107;Peg;Peg9;pG;pG2;preadipocyte factor 1;pref;pref-1;protein delta homolog 1;SC;SCP1;stromal cell derived protein 1;ZO;ZOG.
NCBI Gene Id13386
Protein NameProtein delta homolog 1
Description of TargetMay have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.
Uniprot IDQ09163
Protein Accession #NP_034182.2
Nucleotide Accession #NM_010052.5
Protein Size (# AA)385
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