Catalog No: OKCD02392
Size:96WELLS
Price: $725.00
SKU
OKCD02392
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Datasheets/ManualsPrintable datasheet for DLG4 ELISA Kit (Rat) (OKCD02392)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Discs, Large Homolog 4 (DLG4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Discs, Large Homolog 4 (DLG4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Discs, Large Homolog 4 (DLG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Discs, Large Homolog 4 (DLG4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Discs, Large Homolog 4 (DLG4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Discs, Large Homolog 4 (DLG4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DLG4 Microplate96 Wells (12 x 8 Well strips)
DLG4 Lyophilized Standard2
100X Biotinylated DLG4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.118 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Discs, Large Homolog 4 (DLG4). No significant cross-reactivity or interference between Discs, Large Homolog 4 (DLG4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolDlg4
Gene Full Namediscs large MAGUK scaffold protein 4
Alias Symbolsdiscs, large homolog 4;disks large homolog 4;Dlgh4;postsynaptic density protein 95;PSD95;PSD-95;Sap90;SAP-90;synapse-associated protein 90;synapse-associated protein SAP90.
NCBI Gene Id29495
Protein NameDisks large homolog 4
Description of TargetInteracts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (By similarity).
Uniprot IDP31016
Protein Accession #NP_062567.1
Nucleotide Accession #NM_019621.1
Protein Size (# AA)724
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