DLG4 ELISA Kit (Rat) (OKCA02228)

Catalog No: OKCA02228

Size:96 Wells

Rat DLG4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	100	95-106
  Serum (n=4)	1:2	96	92-100
  Serum (n=4)	1:4	89	85-93
  Serum (n=4)	1:8	94	90-97

• ELISA Kit Principle: Aviva Systems Biology Dlg4 ELISA Kit (Rat) (OKCA02228) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DLG4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DLG4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DLG4 captured in well.
• ELISA Kit Range: 31.25 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	85	80-89
  EDTA plasma (n=4)	98	94-102

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-DLG4 Microplate: 96 Wells (12 x 8 Well strips)
  • DLG4 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated DLG4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 7.81 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DLG4
• Alias Symbols: Dlgh4, PSD95, Sap90
• NCBI Gene Id: 29495
• Protein Name: Disks large homolog 4
• Description of Target: Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
• Uniprot ID: P31016
• Protein Accession #: NP_062567
• Nucleotide Accession #: NM_019621