DLG3 ELISA Kit (Human) (OKCD01009)

Catalog No: OKCD01009 (Formerly GWB-KBBAE7)

Size:96WELLS

Human DLG3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Discs, Large Homolog 3 (DLG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Discs, Large Homolog 3 (DLG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Discs, Large Homolog 3 (DLG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Discs, Large Homolog 3 (DLG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Discs, Large Homolog 3 (DLG3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Discs, Large Homolog 3 (DLG3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DLG3 Microplate: 96 Wells (12 x 8 Well Strips)
  • DLG3 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated DLG3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.115 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Discs, Large Homolog 3 (DLG3). No significant cross-reactivity or interference between Discs, Large Homolog 3 (DLG3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: DLG3
• Gene Full Name: discs large MAGUK scaffold protein 3
• Alias Symbols: discs, large homolog 3;disks large homolog 3;MRX;MRX90;NEDLG;neuroendocrine-DLG;PPP1R82;protein phosphatase 1, regulatory subunit 82;SAP102;synapse-associated protein 102;XLID90;XLMR.
• NCBI Gene Id: 1741
• Protein Name: Disks large homolog 3
• Description of Target: Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling.
• Uniprot ID: Q92796
• Protein Accession #: NP_001159750
• Nucleotide Accession #: NM_001166278.1
• Protein Size (# AA): 817