DLAT Antibody (OAAB21683)

Catalog No: OAAB21683

Size:100ul

Western blot detection of Pyruvate Dehydrogenase E2 in Jurkat, A549, U251, F9, Lncap and Hela cell lysates using Pyruvate Dehydrogenase E2 mouse mAb (OAAB21683) (1:1,000 diluted). Predicted band size: 69KDa. Observed band size: 69KDa.

Immunocytochemistry stain of Hela using Pyruvate Dehydrogenase E2 mouse mAb (OAAB21683) (1:300).

Immunoprecipitation analysis of Hela cell lysates using Pyruvate Dehydrogenase E2 mouse mAb (OAAB21683).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, ICC, IP
• Additional Information: Function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
• Application Info: WB: 1:1000
  ICC: 1:300
  IP: 1:500
• Reference: Totoki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
  Taylor T.D.,et al.Nature 440:497-500(2006).
  Coppel R.L.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:7317-7321(1988).
  Thekkumkara T.J.,et al.FEBS Lett. 240:45-48(1988).
  Hiromasa Y.,et al.J. Biol. Chem. 279:6921-6933(2004).
  

Target Info

• Gene Symbol: DLAT
• Gene Full Name: dihydrolipoamide S-acetyltransferase
• Alias Symbols: E2, PBC, DLTA, PDCE2, PDC-E2
• NCBI Gene Id: 1737
• Description of Target: This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
• Uniprot ID: P10515
• Protein Accession #: NP_001922.2
• Nucleotide Accession #: NM_001931.4
• Molecular Weight: 69 kDa