DKK4 ELISA Kit (Mouse) (OKCD00636)

Catalog No: OKCD00636 (Formerly GWB-KBBAA7)

Size:96WELLS

Mouse Dkk4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	89-98%	94-105%	93-101%	91-98%
  EDTA plasma(n=5)	79-104%	86-102%	97-104%	78-88%
  heparin plasma(n=5)	78-98%	95-102%	88-98%	94-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 4 (DKK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 4 (DKK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 4 (DKK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dickkopf Related Protein 4 (DKK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	95-102	99
  EDTA plasma(n=5)	97-104	101
  heparin plasma(n=5)	92-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dickkopf Related Protein 4 (DKK4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dickkopf Related Protein 4 (DKK4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Dkk4 Microplate: 96 Wells (12 x 8 Well Strips)
  • Dkk4 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Dkk4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.051 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 4 (DKK4). No significant cross-reactivity or interference between Dickkopf Related Protein 4 (DKK4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Dkk4
• Gene Full Name: dickkopf WNT signaling pathway inhibitor 4
• Alias Symbols: dickkopf homolog 4;dickkopf-4;dickkopf-related protein 4;Dkk-4.
• NCBI Gene Id: 234130
• Protein Name: Dickkopf-related protein 4
• Description of Target: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
• Uniprot ID: Q8VEJ3
• Protein Accession #: NP_663567
• Nucleotide Accession #: NM_145592.2
• Protein Size (# AA): 221