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Catalog No: OKCD00766 (Formerly GWB-KBBCX9)
Size:96WELLS
Price: $725.00
SKU
OKCD00766
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Datasheets/ManualsPrintable datasheet for DKK3 ELISA Kit (Rat) (OKCD00766)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)97-105%86-99%94-102%86-102%
EDTA plasma(n=5)79-90%85-93%88-95%98-105%
heparin plasma(n=5)86-93%85-95%78-96%85-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 3 (DKK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 3 (DKK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 3 (DKK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dickkopf Related Protein 3 (DKK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)97-105101
EDTA plasma(n=5)99-105102
heparin plasma(n=5)94-10598
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dickkopf Related Protein 3 (DKK3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dickkopf Related Protein 3 (DKK3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Dkk3 Microplate96 Wells (12 x 8 Well Strips)
Dkk3 Lyophilized Standard2 x 2 ng
100X Biotinylated Dkk3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 14.3 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 3 (DKK3). No significant cross-reactivity or interference between Dickkopf Related Protein 3 (DKK3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDkk3
Gene Full Namedickkopf WNT signaling pathway inhibitor 3
Alias Symbolsdickkopf 3 homolog;dickkopf homolog 3;dickkopf-related protein 3;p29;Reic.
NCBI Gene Id171548
Protein NameDickkopf WNT-signaling pathway inhibitor 3
Description of TargetDickkopf WNT-signaling pathway inhibitor 3
Uniprot IDB1H219
Protein Accession #NP_612528
Nucleotide Accession #NM_138519.2
Protein Size (# AA)348
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