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Catalog No: OKCD02498
Price: $700.00
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Datasheets/ManualsPrintable datasheet for DKK3 ELISA Kit (Mouse) (OKCD02498)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Serum (n=5)96-104%81-103%90-104%93-105%
EDTA Plasma (n=5)98-105%93-101%98-105%79-99%
Heparin Plasma (n=5)90-98%78-89%93-101%88-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 3 (DKK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 3 (DKK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 3 (DKK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dickkopf Related Protein 3 (DKK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)84-10296
EDTA Plasma (n=5)78-10386
Heparin Plasma(n=5)80-10196
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dickkopf Related Protein 3 (DKK3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dickkopf Related Protein 3 (DKK3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
Anti-DKK3 Microplate96 Wells (12 x 8 Well strips)
DKK3 Lyophilized Standard2
100X Biotinylated DKK3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity0.067 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 3 (DKK3). No significant cross-reactivity or interference between Dickkopf Related Protein 3 (DKK3) and analogues was observed.

A combined bioinformatics, experimental and clinical approach to identify novel cardiac-specific heart failure biomarkers: is Dickkopf-3 (DKK3) a possible candidate?. Eur J Heart Fail. 22, 2065-2074 (2020). 32809235

Bone secreted factors induce cellular quiescence in prostate cancer cells. Sci Rep. 9, 18635 (2019). 31819067

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolDkk3
Gene Full Namedickkopf WNT signaling pathway inhibitor 3
Alias SymbolsAW061014;C87148;dickkopf homolog 3;dickkopf-3;dickkopf-related protein 3;dkk-3;mDkk-3.
NCBI Gene Id50781
Protein NameDickkopf-related protein 3
Description of TargetAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
Uniprot IDQ9QUN9
Protein Accession #NP_056629.1
Nucleotide Accession #NM_015814.2
Protein Size (# AA)349
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