Catalog No: OKCD02498
Size:96 Wells
Price: $700.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration3 Hours
ELISA Kit Linearity
Serum (n=5)96-104%81-103%90-104%93-105%
EDTA Plasma (n=5)98-105%93-101%98-105%79-99%
Heparin Plasma (n=5)90-98%78-89%93-101%88-96%
ELISA Kit PrincipleAviva Systems Biology Dkk3 ELISA Kit (Mouse) (OKCD02498) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dkk3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Dkk3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Dkk3 captured in well.
ELISA Kit Range0.156-10 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)84-10296
EDTA Plasma (n=5)78-10386
Heparin Plasma(n=5)80-10196
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level DKK3 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level DKK3 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
ELISA Kit Component
Anti-DKK3 Microplate96 Wells (12 x 8 Well strips)
DKK3 Lyophilized Standard2
100X Biotinylated DKK3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity0.067 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 3 (DKK3). No significant cross-reactivity or interference between Dickkopf Related Protein 3 (DKK3) and analogues was observed.

A combined bioinformatics, experimental and clinical approach to identify novel cardiac-specific heart failure biomarkers: is Dickkopf-3 (DKK3) a possible candidate?. Eur J Heart Fail. 22, 2065-2074 (2020). 32809235

Bone secreted factors induce cellular quiescence in prostate cancer cells. Sci Rep. 9, 18635 (2019). 31819067

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolDKK3
Gene Full Namedickkopf WNT signaling pathway inhibitor 3
Alias Symbolsdkk-3, C87148, mDkk-3, AW061014
NCBI Gene Id50781
Protein NameDickkopf-related protein 3
Description of TargetAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Uniprot IDQ9QUN9
Protein Accession #NP_056629.1
Nucleotide Accession #NM_015814.2
Protein Size (# AA)349
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