DKK3 ELISA Kit (Mouse) (OKCD02498)

Catalog No: OKCD02498

Size:96 Wells

Mouse DKK3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 3 Hours
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-104%	81-103%	90-104%	93-105%
  EDTA Plasma (n=5)	98-105%	93-101%	98-105%	79-99%
  Heparin Plasma (n=5)	90-98%	78-89%	93-101%	88-96%

• ELISA Kit Principle: Aviva Systems Biology Dkk3 ELISA Kit (Mouse) (OKCD02498) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dkk3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Dkk3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Dkk3 captured in well.
• ELISA Kit Range: 0.156-10 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	84-102	96
  EDTA Plasma (n=5)	78-103	86
  Heparin Plasma(n=5)	80-101	96

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level DKK3 was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level DKK3 was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10% (n=20)
  Inter-Assay CV: <12% (n=24)
• ELISA Kit Component:
  • Anti-DKK3 Microplate: 96 Wells (12 x 8 Well strips)
  • DKK3 Lyophilized Standard: 2
  • 100X Biotinylated DKK3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.067 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 3 (DKK3). No significant cross-reactivity or interference between Dickkopf Related Protein 3 (DKK3) and analogues was observed.
• Publications:

  A combined bioinformatics, experimental and clinical approach to identify novel cardiac-specific heart failure biomarkers: is Dickkopf-3 (DKK3) a possible candidate?. Eur J Heart Fail. 22, 2065-2074 (2020). 32809235

  Bone secreted factors induce cellular quiescence in prostate cancer cells. Sci Rep. 9, 18635 (2019). 31819067

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DKK3
• Gene Full Name: dickkopf WNT signaling pathway inhibitor 3
• Alias Symbols: dkk-3, C87148, mDkk-3, AW061014
• NCBI Gene Id: 50781
• Protein Name: Dickkopf-related protein 3
• Description of Target: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
• Uniprot ID: Q9QUN9
• Protein Accession #: NP_056629.1
• Nucleotide Accession #: NM_015814.2
• Protein Size (# AA): 349